Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR

doi:10.1007/s00253-022-11822-4

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	Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR
	Tao, Yile 1,2; Yue, Yang 1,2; Qiu, Guangyu 1,2; Ji, Zheng 3; Spillman, Martin 1,2; Gai, Zhibo 4; Chen, Qingfa 5; Bielecki, Michel 6; Huber, Michael 7; Trkola, Alexandra 7; Wang, Qiyuan 8,9; Cao, Junji 8,9; Wang, Jing 1,2
通讯作者	Wang, Jing(jing.wang@ifu.baug.ethz.ch)
	2022-02-26
发表期刊	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN	0175-7598
页码	12
摘要	The pandemic of coronavirus disease 2019 (COVID-19) continues to threaten public health. For developing countries where vaccines are still in shortage, cheaper alternative molecular methods for SARS-CoV-2 identification can be crucial to prevent the next wave. Therefore, 14 primer sets recommended by the World Health Organization (WHO) was evaluated on testing both clinical patient and environmental samples with the gold standard diagnosis method, TaqMan-based RT-qPCR, and a cheaper alternative method, SYBR Green-based RT-qPCR. Using suitable primer sets, such as ORF1ab, 2019_nCoV_N1 and 2019_nCoV_N3, the performance of the SYBR Green approach was comparable or better than the TaqMan approach, even when considering the newly dominating or emerging variants, including Delta, Eta, Kappa, Lambda, Mu, and Omicron. ORF1ab and 2019_nCoV_N3 were the best combination for sensitive and reliable SARS-CoV-2 molecular diagnostics due to their high sensitivity, specificity, and broad accessibility.
关键词	SARS-CoV-2 COVID-19 SYBR Green TaqMan probe RT-qPCR
DOI	10.1007/s00253-022-11822-4
关键词[WOS]	POLYMERASE-CHAIN-REACTION ; COVID-19 ; PCR
收录类别	SCI ; SCI
语种	英语
资助项目	China Scholarship Council[201906875037] ; China Scholarship Council[201706010410]
WOS研究方向	Biotechnology & Applied Microbiology
项目资助者	China Scholarship Council
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000761867000005
出版者	SPRINGER
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ieecas.cn/handle/361006/17473
专题	粉尘与环境研究室第四纪科学与全球变化卓越创新中心
通讯作者	Wang, Jing
作者单位	1.Swiss Fed Inst Technol, Inst Environm Engn, CH-8093 Zurich, Switzerland 2.Swiss Fed Labs Mat Sci & Technol, Lab Adv Analyt Technol, Empa, CH-8600 Dubendorf, Switzerland 3.Shaanxi Normal Univ, Sch Geog & Tourism, Xian 710119, Peoples R China 4.Univ Zurich, Univ Hosp Zurich, Dept Clin Pharmacol & Toxicol, CH-8091 Zurich, Switzerland 5.Liaocheng Univ, Inst Tissue Engn & Regenerat Med, Liaocheng 252000, Shandong, Peoples R China 6.Univ Zurich, Epidemiol Biostat & Prevent Inst, CH-8091 Zurich, Switzerland 7.Univ Zurich, Inst Med Virol, CH-8057 Zurich, Switzerland 8.Chinese Acad Sci, Inst Earth Environm, Key Lab Aerosol Chem & Phys, State Key Lab Loess & Quaternary Geol, Xian 710061, Peoples R China 9.CAS Ctr Excellence Quaternary Sci & Global Change, Xian 710061, Peoples R China
推荐引用方式 GB/T 7714	Tao, Yile,Yue, Yang,Qiu, Guangyu,et al. Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2022:12.
APA	Tao, Yile.,Yue, Yang.,Qiu, Guangyu.,Ji, Zheng.,Spillman, Martin.,...&Wang, Jing.(2022).Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,12.
MLA	Tao, Yile,et al."Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2022):12.